Question

why do we need to add buffer into the master mix? a) to increase master mix volume b) to remove color from the master mix c) to stabilize reaction ph d) to stabilize master mix temperature

Explanation:

Buffers resist changes in pH. In a master - mix, maintaining a stable pH is crucial for the proper functioning of enzymes and other components involved in reactions. So, the main reason to add a buffer to a master - mix is to stabilize the reaction pH.

Answer:

c) To stabilize reaction pH