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Question
what would happen if the gel was run for too long?
○ nothing would happen. the bands would just stop in the middle of the gel no matter how long it’s run.
○ the sample bands would not separate far enough to see reliable results.
○ the sample bands would move too far and leave the bottom of the gel.
Gel electrophoresis separates molecules (e.g., DNA) by size/charge under an electric field. Running the gel too long means molecules continue migrating until they exit the gel's bottom, as there's no barrier to stop them once they reach the end.
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The sample bands would move too far and leave the bottom of the gel.