Question

question 6 / 10
a reaction tube is set up at 37 degrees celsius with twice as much substrate as enzyme. the ph level of the solution is 5. the reaction rate is measured. which of the following changes will not affect the rate of the reaction?
a. increasing the ph level
b. increasing the temperature
c. increasing the enzyme concentration
d. increasing the substrate concentration

Explanation:

Enzymes have an optimal pH level, temperature, and enzyme - substrate interaction. When there is already twice as much substrate as enzyme, further increasing the substrate concentration (option D) will not affect the reaction rate as the enzyme is already saturated with substrate. Increasing pH (A) can affect enzyme activity as enzymes have an optimal pH. Increasing temperature (B) can affect enzyme activity (up to the denaturation point). Increasing enzyme concentration (C) can increase the reaction rate as more enzymes can act on the substrate.

Answer:

D. increasing the substrate concentration