Question

part 1 (1 point) the frap technique occurs in a series of steps. select every statement that correctly describes a step in the frap procedure. choose one or more: a. the molecule of interest is fluorescently labeled. b. the relative mobility of the fluorescently labeled molecule is measured. c. all fluorescent molecules in the cell are irreversibly bleached. d. the speed of repair of the fluorescent marker is measured.

Explanation:

FRAP (Fluorescence Recovery After Photobleaching) first labels the molecule of interest with a fluorescent marker. Then, the relative mobility of the labeled molecule is measured by observing the recovery of fluorescence after a region is photobleached. The speed of repair of the fluorescent marker (fluorescence recovery) is also measured. Not all fluorescent molecules in the cell are irreversibly bleached; only a specific region is bleached.

Answer:

A. The molecule of interest is fluorescently labeled.
B. The relative mobility of the fluorescently labeled molecule is measured.
D. The speed of repair of the fluorescent marker is measured.