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Question
how is the banding pattern made easier to see in gel electrophoresis?
a. bright light is applied
b. using a microscope
c. a dye or stain is applied that glows under a uv light
d. nothing, it can be seen easily with the naked eye.
question 7
1 pts
what are some applications of gel electrophoresis in molecular biology?
a. it is only used for dna fingerprinting
b. including dna fingerprinting (the name for dna tests), genotyping (genetic testing), and the confirmation of successful dna amplification in polymerase chain reaction (pcr) (a technique to get more dna to use for medical diagnosis, to make dna tests more accurate, to prepare for cloning experiments, etc)
c. it is used to create human clones
d. it is only used in crime scene investigations
For the first question: In gel electrophoresis, a dye or stain (like ethidium bromide) that glows under UV light is used to visualize the banding pattern. Bright light or naked - eye observation is not sufficient as the DNA/RNA/protein samples are not visible without staining. A microscope is not typically used in the standard visualization of gel electrophoresis bands in this context.
For the second question: Gel electrophoresis has multiple applications. It is not only used for DNA fingerprinting (so A and D are wrong). It is also not used to create human clones (C is wrong). It is used in DNA fingerprinting, genotyping, and confirming successful DNA amplification in PCR.
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First question: C. a dye or stain is applied that glows under a UV light
Second question: B. including DNA fingerprinting (the name for DNA tests), genotyping (genetic testing), and the confirmation of successful DNA amplification in polymerase chain reaction (PCR) (a technique to get more DNA to use for medical diagnosis, to make DNA tests more accurate, to prepare for cloning experiments, etc)