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Question
- what is the name of the molecule that binds to proteins producing a blue color? it was used in the bradford assay. at what wavelength does this molecule absorb? a. 410 nm b. 595 nm
Brief Explanations
In the Bradford assay, Coomassie Brilliant Blue binds to proteins. The bound form of this dye has a maximum absorbance at 595 nm. This property is used to quantify proteins as the absorbance at this wavelength is proportional to the protein concentration.
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Coomassie Brilliant Blue; b. 595 nm