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Question
question 1
which of the following utensils is used to inoculate motility agar?
inoculating needle
inoculating loop
sterile cotton-tipped applicator
inoculating brush
Identify the inoculation method for motility agar
Motility test medium is a semi-solid agar designed to detect bacterial motility. To perform this test, a straight line must be stabbed into the center of the medium without shearing or spreading the agar.
Evaluate the appropriate tool
An inoculating needle is a straight wire used to make a clean, single-line stab into semi-solid media. An inoculating loop, cotton-tipped applicator, or brush would disrupt the agar structure and make it impossible to accurately determine if bacterial growth is spreading away from a single stab line.
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- inoculating needle (Correct answer)
- inoculating loop
- sterile cotton-tipped applicator
- inoculating brush