Question

question 14
select all the correct statements related to this test.
hydrogen peroxide is the substrate for this enzyme
the bubbles seen are hygrogen
the bubbles seen are oxygen
cytochrome c is the substrate for this test
this is the test for catalase enzyme
this is the test for oxidase enzyme

Explanation:

1. Hydrogen peroxide as substrate: Catalase enzyme acts on hydrogen peroxide ($\ce{H_2O_2}$) as its substrate, breaking it down. So this statement is correct.
2. Bubbles as hydrogen: The reaction of catalase with $\ce{H_2O_2}$ produces oxygen ($\ce{O_2}$) gas (bubbles), not hydrogen. So this is incorrect.
3. Bubbles as oxygen: Catalase decomposes $\ce{H_2O_2}$ into water and oxygen ($\ce{2H_2O_2 \xrightarrow{\text{catalase}} 2H_2O + O_2}$), so the bubbles are oxygen. Correct.
4. Cytochrome c as substrate: Cytochrome c is a substrate for oxidase enzyme, not for the catalase test shown here. Incorrect.
5. Test for catalase enzyme: The presence of bubbles (oxygen) from $\ce{H_2O_2}$ breakdown indicates the catalase test. Correct.
6. Test for oxidase enzyme: Oxidase test involves a different reaction (e.g., using cytochrome c) and does not produce oxygen bubbles from $\ce{H_2O_2}$. Incorrect.

Answer:

• Hydrogen peroxide is the substrate for this enzyme
• The bubbles seen are oxygen
• This is the test for catalase enzyme