Question

question 7 of 10
a scientist is trying to find a method to artificially produce a protein that is
found in a pigs kidney. what would be the first step of this process?
a. insert dna into a sample of bacteria.
b. find all the mrna produced by the kidney.
c. mix a cdna probe with kidney dna.
d. use reverse transcriptase to make cdna.

Explanation:

To artificially produce a protein, the first step is to identify the mRNA that codes for that protein. mRNA is transcribed from the DNA that contains the gene for the protein. Once the mRNA is found, it can be used to make cDNA (using reverse transcriptase, which is step D, but that's not the first step). Inserting DNA into bacteria (step A) is later in the process (after the gene is isolated). Mixing a cDNA probe with kidney DNA (step C) is also a later step (probes are used to identify specific DNA sequences, but you first need to have the mRNA to know what you're looking for in terms of the gene). So the first step is to find the mRNA produced by the kidney (as it carries the coding information for the protein).

Answer:

B. Find all the mRNA produced by the kidney.