Question

name_______per__date______
1.1.6 dna analysis using ge- 12 points
directions: as you read through pltw 1.1.6 dna analysis, answer the following questions. be sure to go through the slides in gel electrophoresis demonstration to help you understand how this process works.

1. what do enzymes do?
2. what is the process called that we will use to separate and compare dna?
3. what do we \see\ to know that the dna has run across the agarose gel?
4. why are we going to test dna samples only from anna, eric, and dominique? why arent we testing samples from the other 3 persons of interest?
5. which pieces of dna travel faster, and therefore further, across the gel?
6. why does dna migrate towards the positive pole?
7. what are rflps? tell what this stands for and what they are.
8. you should now understand how to

ead the gel\ and are now ready to analyze the dna samples related to the anna garcia case. use the image provided in todays module to see how the gel would look.
sketch the gel making sure you label all lanes below.
(picture found in our module using link)
summarize your observations and conclusions from these results. give some description of how the gel results support your conclusions.

Explanation:

1. Enzymes act as biological catalysts to speed up chemical reactions (e.g., DNA cutting by restriction enzymes).
2. Gel electrophoresis is used to separate and compare DNA fragments.
3. Fluorescent or UV-visible DNA stains (e.g., ethidium bromide) show DNA migration.
4. The question refers to a specific case (Anna Garcia) where Anna, Eric, and Dominique are key persons of interest; other individuals may lack relevant evidence linking them to the case.
5. Smaller DNA fragments travel faster and farther through the gel pores.
6. DNA is negatively charged (due to phosphate groups), so it moves toward the positive pole in an electric field.
7. RFLPs stand for Restriction Fragment Length Polymorphisms—DNA fragments of varying lengths produced by cutting DNA with restriction enzymes, unique to individuals.
8. (Sketch requires the module image; observations/conclusions depend on matching DNA band patterns between samples, e.g., linking a suspect’s DNA to evidence.)

Answer:

1. Speed up chemical reactions as biological catalysts
2. Gel electrophoresis
3. Fluorescent/UV-visible DNA stains
4. They are key persons of interest in the Anna Garcia case (others lack relevant links)
5. Smaller DNA fragments
6. DNA is negatively charged
7. Restriction Fragment Length Polymorphisms; unique DNA fragment lengths from restriction enzyme cuts
8. (Sketch: Label lanes per module image; Observations/conclusions: Match band patterns to link samples)