Question

labster pcr post-lab questions
1002

1. where is the dna going to come from in the suspect’s blood that was found at the crime scene?
2. what part of the dna sequence are we going to amplify by pcr and why?
3. what are we going to use to target this specific region?
4. what does pcr stand for?
5. what do we have to do to the sample before running pcr?
6. what is the enzyme the uses the nucleotides to build the new dna strands?
7. why is it important to change the pipette tip each time you pipette a new liquid sample?
8. at what temperature do the dna stands break apart to expose the nucleotides on single strands and what is this step called?

Explanation:

Answer:

White blood cells (WBCs)
Short Tandem Repeats (STRs) as they are highly variable among individuals
Primers
Polymerase Chain Reaction
Extract and purify the DNA
Taq polymerase
To prevent cross - contamination
95°C, denaturation