Question

drag each tile to the correct box.

rna interference is a mechanism of gene regulation in eukaryotes. arrange the steps by which rnai carries out gene regulation in the correct order.

tiles:

• the sirna binds to a special protein, which degrades one strand.
• one strand of sirna associates with the protein to form an rnase complex.
• the enzyme dicer cleaves dsrna to form small interfering rna (sirna).
• the rnase complex binds to a specific mrna and degrades it.

Explanation:

Identify the initial cleavage step

The process begins with the enzyme Dicer cleaving double-stranded RNA (dsRNA) into small interfering RNA (siRNA).

Determine the protein binding and strand degradation step

Next, the newly formed siRNA binds to a specialized protein complex, which degrades one of the two strands of the siRNA.

Identify the formation of the active RNase complex

The remaining single strand of siRNA associates with the protein complex to form the active RNase complex (RISC).

Determine the final mRNA targeting and degradation step

Finally, this active RNase complex binds to a complementary target mRNA sequence and degrades it, preventing translation.

Answer:

1. The enzyme dicer cleaves dsRNA to form small interfering RNA (siRNA).
2. The siRNA binds to a special protein, which degrades one strand.
3. One strand of siRNA associates with the protein to form an RNase complex.
4. The RNase complex binds to a specific mRNA and degrades it.