QUESTION IMAGE
Question
collect data: use the four available tests to find the nutritional content of samples on the table below. (sample a has been done for you as an example.) record the results.
| carbohydrate tests | protein test | lipids test | test results – are these nutrients present? | |||||
| food sample | benedict test | lugol test | biuret test | sudan red test | mono - saccharides | starches | proteins | lipids |
| a | + | - | - | - | yes | no | no | no |
| b | ||||||||
| f | ||||||||
| h | ||||||||
| i | ||||||||
| m |
which sample came from each person? how do you know?
Step1: Recall Nutrient Test Principles
- Benedict test: Detects monosaccharides (e.g., glucose). Positive = color change (e.g., blue to orange/red).
- Lugol test: Detects starches. Positive = blue-black color.
- Biuret test: Detects proteins. Positive = purple color.
- Sudan Red test: Detects lipids. Positive = red-stained droplets.
Step2: Apply Tests to Samples (Example Workflow)
For a food sample (e.g., Sample B):
- Perform Benedict test: Observe color. If +, monosaccharides = Yes; else No.
- Perform Lugol test: Observe color. If + (blue-black), starches = Yes; else No.
- Perform Biuret test: Observe color. If + (purple), proteins = Yes; else No.
- Perform Sudan Red test: Observe droplets. If + (red), lipids = Yes; else No.
(Note: Since no specific sample data is filled, the process involves conducting each test and recording results as per the color/observation changes.)
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The process involves using each test (Benedict, Lugol, Biuret, Sudan Red) on each food sample, observing color changes/droplets, and recording "Yes" or "No" for each nutrient (monosaccharides, starches, proteins, lipids) based on test results. For example, if Sample B's Benedict test is +, monosaccharides = Yes; if Lugol test is -, starches = No, etc. (Actual results depend on the food sample's composition and test observations.)