Question

which of the following enzyme is needed to reseal breaks in the sugar - phosphate backbone during replication?
multiple choice
dna polymerase
rna polymerase
helicase
dna ligase
topoisomerase

Explanation:

• DNA polymerase: Synthesizes new DNA strands by adding nucleotides.
• RNA polymerase: Involved in transcription (synthesis of RNA from DNA template).
• Helicase: Unwinds the DNA double - helix.
• DNA ligase: Catalyzes the formation of phosphodiester bonds to seal breaks (nicks) in the sugar - phosphate backbone of DNA, which is crucial during replication (e.g., joining Okazaki fragments on the lagging strand).
• Topoisomerase: Relieves torsional stress in the DNA molecule during processes like replication.

Answer:

DNA ligase