Question

what is your prediction for this experiment?

a. the organism will grow randomly at different temperatures.
b. the organism will grow evenly at all temperatures.
c. the organism will grow only at one temperature.
d. the organism will grow within a range of temperatures.

what is your strategy?

a. inoculate a series of broths and incubate at different temperatures to compare growth.
b. inoculate a broth and incubate at room temperature to start growth. if it grows, inoculate more tubes from it, and incubate at temperatures colder than room temperature to look for psychrophiles.
c. inoculate a broth and incubate at the highest temperature to check for thermophiles. if it does not grow, inoculate more tubes from it and incubate at temperatures colder than room temperature to look for psychrophiles.

Explanation:

Analyze the prediction question

Microorganisms have defined minimum, optimum, and maximum growth temperatures. Therefore, an organism will grow within a specific range of temperatures rather than randomly, evenly, or at only one single temperature. This matches option D.

Analyze the strategy question

To determine the temperature range and optimum growth temperature of an organism, the standard experimental approach is to inoculate a series of broths and incubate them at different temperatures to compare growth. This matches option A.

Answer:

Question 1

• A. The organism will grow randomly at different temperatures.
• B. The organism will grow evenly at all temperatures.
• C. The organism will grow only at one temperature.
• D. The organism will grow within a range of temperatures. (Correct answer)

Question 2

• A. Inoculate a series of broths and incubate at different temperatures to compare growth. (Correct answer)
• B. Inoculate a broth and incubate at room temperature to start growth. If it grows, inoculate more tubes from it, and incubate at temperatures colder than room temperature to look for psychrophiles.
• C. Inoculate a broth and incubate at the highest temperature to check for thermophiles. If it does not grow, inoculate more tubes from it and incubate at temperatures colder than room temperature to look for psychrophiles.