Question

what would happen if you set a micropipette outside its designated volume range? select all answers that apply.

a. nothing would happen, the volumes on the micropipettes are are suggested volumes only.

b. it would lead to inaccurate volume measurements.

c. it would damage or contaminate the micropipette

d. it would allow the micropipette to collect a larger volume assuming you attached a larger disposable tip.

Explanation:

• Option a: Micropipette volume ranges are not just suggestions. They are based on calibration and engineering for accuracy. So, this option is incorrect.
• Option b: When set outside the range, the internal mechanism (like piston - spring system) is not calibrated for that volume. This leads to inaccurate volume measurements as the displacement of the piston (which determines volume) is not in the correct proportion for the set value.
• Option c: Forcing the micropipette outside its range (e.g., over - extending the piston) can damage the internal components (such as the seal, spring, or piston itself). Also, if the volume is too low, liquid can be drawn into parts of the pipette where it should not be, causing contamination.
• Option d: The volume range is determined by the internal mechanism of the micropipette, not just the tip. A larger tip does not change the volume - measuring ability of the pipette's internal components. So, this option is incorrect.

Answer:

B. It would lead to inaccurate volume measurements, C. It would damage or contaminate the micropipette