Question

1. what is the depth of field? 4. what is the field of view? 5. images observed using the light microscope are inverted and reversed. what does this mean? 6. explain why an image must be centered in the field of view when using low power before moving to a higher power magnification. 7. describe how the field of view and the amount of available light change when going from high to low power objectives.

Explanation:

1. Depth of field is the range of distance in a specimen that appears acceptably in - focus. It is affected by aperture size, focal length, and magnification. Smaller apertures increase depth of field.
2. Field of view is the circular area visible through the microscope eyepiece. It is measured as the diameter of the circular area and decreases as magnification increases.
3. Inverted and reversed means that the image seen through the light - microscope is upside - down and left - right reversed compared to the actual specimen. This is due to the way light rays are bent by the lenses in the microscope.
4. When moving from low power to high power magnification, the field of view decreases. If the image is not centered at low power, it may move out of the field of view at high power and be lost.
5. When going from high to low power objectives, the field of view increases (more of the specimen is visible), and the amount of available light increases because the low - power objective has a larger aperture and allows more light to enter.

Answer:

1. The range of distance in a specimen that appears acceptably in - focus.
2. The circular area visible through the microscope eyepiece, measured as its diameter.
3. The image is upside - down and left - right reversed compared to the actual specimen.
4. To prevent the image from moving out of the field of view as magnification increases.
5. The field of view increases and the amount of available light increases.