Question

serial dilutions: 8-channel micropipette hri

1. use 2 well plates
2. fill one of the well plates with di water from row a to g
3. fill one wells with the stock solution
4. use a two - fold dilution filling each well with 50ul of di water
5. fill the first well with 100 ul of the stock solution
6. transfer 50ul from well 1 to well 2 and repeat the process to row h
7. calculate the dilution and concentration. the stock solution has a value of 100 mg/ml

well row	concentration	dilution
b	50/100	50/100 = 1/2 = 0.5
c	0.25 mg/ml	25/50 = 1/2 = 0.25
d	12.5	12.3/25 = 1/2 = 0.12
e	6.25
f
g

1. find the grams of

Explanation:

Step1: Define dilution factor

Two-fold dilution means each step dilutes by 1/2.

Step2: Calculate Row A

Stock solution: Concentration = 100 mg/mL, Dilution = 1 (undiluted).

Step3: Calculate Row B

Concentration = 100 × 1/2 = 50 mg/mL; Dilution = 1/2 = 0.5.

Step4: Calculate Row C

Concentration = 50 × 1/2 = 25 mg/mL; Dilution = 1/2 × 1/2 = 1/4 = 0.25.

Step5: Calculate Row D

Concentration = 25 × 1/2 = 12.5 mg/mL; Dilution = 1/4 × 1/2 = 1/8 = 0.125.

Step6: Calculate Row E

Concentration = 12.5 × 1/2 = 6.25 mg/mL; Dilution = 1/8 × 1/2 = 1/16 = 0.0625.

Step7: Calculate Row F

Concentration = 6.25 × 1/2 = 3.125 mg/mL; Dilution = 1/16 × 1/2 = 1/32 = 0.03125.

Step8: Calculate Row G

Concentration = 3.125 × 1/2 = 1.5625 mg/mL; Dilution = 1/32 × 1/2 = 1/64 = 0.015625.

Answer:

Well Row	Concentration (mg/mL)	Dilution
B	50	1/2 = 0.5
C	25	1/4 = 0.25
D	12.5	1/8 = 0.125
E	6.25	1/16 = 0.0625
F	3.125	1/32 = 0.03125
G	1.5625	1/64 = 0.015625