Question

a restriction enzyme cuts dna 1000 base pairs long at the following recognition sites: 300 base pairs, 450 base pairs and 650 base pairs (see below). what is the size of the restriction fragment indicated by the arrow? 350 bp 150 bp 200 bp 1000 bp 300 bp

Explanation:

Step1: List recognition sites in order

Sorted sites: 300 bp, 450 bp, 650 bp

Step2: Calculate fragment sizes

Fragments: 300-0=300 bp, 450-300=150 bp, 650-450=200 bp, 1000-650=350 bp

Step3: Match arrow (implied 650-450)

Fragment size: 200 bp

Answer:

C. 200 bp