Question

the replicated chromosomes are separated by spindle fibers during
prophase.
metaphase.
anaphase.
telophase.

there is no doubt that a chemical reaction has occurred if
there has been an overall volume change.
a new substance has been formed.
there has been a change of state.
color has been changed.

Explanation:

• For the first question: In anaphase of cell division, spindle fibers pull the replicated chromosomes (sister chromatids) apart to opposite poles of the cell. In prophase, chromosomes condense and spindle fibers start to form. In metaphase, chromosomes line up at the equator. In telophase, nuclear envelopes reform.
• For the second question: The key characteristic of a chemical reaction is the formation of a new substance. A change in volume, state, or color can also occur in physical changes (e.g., water boiling - change of state, mixing two colored solutions - color change, gas expansion - volume change).

Answer:

1. anaphase
2. a new substance has been formed.