Question

question 1
david pribnow studied the base sequences of promoters in bacteria and bacterial viruses. he found two conserved regions in these promoters (the -10 box and the -35 box). these two regions of the promoter
a signal the initiation site
b bind the sigma subunit that is associated with rna polymerase
c attach the correct nucleotide triphosphate to the template dna strand
d separate the two dna strands

Explanation:

In bacteria, the promoter regions (-10 box and -35 box) are recognized by the sigma subunit of RNA polymerase. The sigma subunit helps in the binding of RNA polymerase to the promoter. The initiation site is a specific nucleotide where transcription starts, which is not the function of these promoter boxes. Nucleotide triphosphates are attached by RNA polymerase during elongation (not related to promoter boxes). DNA strand separation is mainly by RNA polymerase (with help from sigma initially but not the main role of -10 and -35 boxes as per their conserved binding function).

Answer:

B. bind the sigma subunit that is associated with RNA polymerase