Question

question 43
2 points
1 2 3 4
antigen (like virus, aflatoxin,...)
antibody with attached (conjugated) enzyme
substrate transformed into a product by the enzyme
is a secondary antibody needed for this example?
a yes
b no
last saved 9:22:34 pm

Explanation:

In this immunoassay (like ELISA), the antibody used has a conjugated enzyme (seen as the antibody with attached enzyme). Secondary antibodies are used when the primary antibody isn't conjugated, but here the primary (or the antibody interacting with antigen) is already conjugated. So no secondary antibody is needed.

Answer:

B. No