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Question
question 26
1 pts
which of the following is not necessary to execute a successful pcr?
o short single - stranded dna primers
o all four dna bases
o dna polymerase
o repair enzymes
PCR (Polymerase Chain Reaction) requires short single - stranded DNA primers to initiate DNA synthesis, all four DNA bases (dNTPs - deoxyribonucleoside triphosphates) as building blocks for new DNA strands, and DNA polymerase (usually a heat - stable one like Taq polymerase) to catalyze the addition of nucleotides. Repair enzymes are not necessary for the basic PCR process. PCR is mainly about amplifying a specific DNA region through repeated cycles of denaturation, annealing (of primers), and extension (by DNA polymerase), and DNA repair is not a part of this core amplification mechanism.
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repair enzymes