Question

question 10
1 pts
in the lab you did on editing lacz in e. coli, the bacteria that were transformed with the plzdonor plasmid were missing ______.

• a way to do dna repair
• a way to guide cas9 to lacz
• a way to do blue-white selection
• a piece of dna to swap for lacz

Explanation:

The pLZDonor plasmid typically provides the donor DNA template for homologous recombination with the lacZ gene during editing. However, it lacks the guide RNA (gRNA) necessary to direct the Cas9 enzyme to the specific lacZ target sequence. DNA repair mechanisms, blue-white selection components, and the donor DNA swap template are either inherent to the bacterial system or provided by the plasmid, respectively.

Answer:

B. a way to guide Cas9 to lacZ