Question

put the steps for sickle-cell anemia gene therapy in order.
1 patient receives chemotherapy to reduce stem cells
2 infect stem cells with genetically engineered virus containing healthy beta-globin
3 genetically engineered stem cells reintroduced to patient
4 stem cells incorporate healthy gene
5 patient takes medication to boost stem cell production
6 stem cells grow to large numbers in vitro
7 harvesting of mutated hematopoietic stem cells

○ 5,6,7,2,4,1,3
○ 5,7,2,4,6,1,3
○ 5,1,7,2,4,6,3
○ 1,2,4,7,3,5,6
○ 1,2,3,4,5,6,7

Explanation:

Step1: Patient takes medication to boost stem cell production

This step increases the number of stem cells available for the process.

Step2: Patient receives chemotherapy to reduce stem cells

Chemotherapy is used to prepare the patient's body for the new stem cells.

Step3: Harvesting of mutated hematopoietic stem cells

The existing stem cells (which are mutated in sickle - cell anemia) are collected.

Step4: Infect stem cells with genetically engineered virus containing healthy beta - globin

The virus is used as a vector to introduce the healthy gene.

Step5: Stem cells incorporate healthy gene

The stem cells take up the healthy gene from the virus.

Step6: Stem cells grow to large numbers in vitro

The genetically modified stem cells are grown in a laboratory setting.

Step7: Genetically engineered stem cells reintroduced to patient

The new, healthy stem cells are put back into the patient's body.

Answer:

5, 1, 7, 2, 4, 6, 3