Question

part d
given a template strand of 3-atgctiggaca-5 and a partially made complementary strand containing only 5-tac-3, what would be the sequence of the new strand of dna (including the 5-tac-3) if the only additional nucleotides available to dna polymerase were those containing the bases g, a, and c?
o 5-tacgaacc-3
o 5-tac-3; all four nucleotides are required for dna polymerase to function
o 5-gaacc-3
o 3-tacgaacctgt-5
part e
what materials does dna polymerase require in order to synthesize a complete strand of dna?
select all that apply.
atp
all four deoxyribonucleotides triphosphates (containing a, c, t, or g)
single-stranded dna template
3-oh end of the new dna strand
inorganic phosphate

Explanation:

Part D

Step1: Determine the complementary base pairing rules

In DNA, A (adenine) pairs with T (thymine), and C (cytosine) pairs with G (guanine). The template strand is \(3' - ATGCTTGACA - 5'\). We need to build the new strand in the \(5' - 3'\) direction.

Step2: Pair the bases for the given partial strand \(5' - TAC - 3'\)

For the \(T\) in \(5' - TAC - 3'\), the complementary base on the template strand (which is read \(3' - 5'\)) is \(A\). For \(A\), it is \(T\), and for \(C\), it is \(G\). Now, looking at the template strand \(3' - ATGCTTGACA - 5'\), after \(3' - ATG\) (complementary to \(5' - TAC - 3'\)), the next bases on the template are \(CTTGACA\). Pairing these: \(C\) pairs with \(G\), \(T\) with \(A\), \(T\) with \(A\), \(G\) with \(C\), \(A\) with \(T\), \(C\) with \(G\), \(A\) with \(T\). So the full new strand (including \(5' - TAC - 3'\)) is \(5' - TACGAACC - 3'\)

Part E

Step1: Recall the requirements for DNA polymerase

DNA polymerase requires a single - stranded DNA template (to read the sequence), the \(3'-OH\) end of the new DNA strand (to add new nucleotides to, as it synthesizes in the \(5' - 3'\) direction), and all four deoxyribonucleotide triphosphates (dNTPs: containing A, C, T, or G) as the building blocks. Inorganic phosphate is a by - product of the reaction (from the hydrolysis of dNTPs to add the nucleotide to the growing strand), and ATP is not a direct substrate for DNA polymerase in DNA synthesis.

Answer:

• Part D: \(5' - TACGAACC - 3'\)
• Part E: \(3 - OH\) end of the new DNA strand, Single - stranded DNA template, All four deoxyribonucleotides triphosphates (containing A, C, T, or G)