Question

part c - atp synthesis in glycolysis: substrate-level phosphorylation
the atp that is generated in glycolysis is produced by substrate-level phosphorylation, a very different mechanism than the one used to produce atp during oxidative
group to another molecule.
sort the statements into the appropriate bin depending on whether or not they correctly describe some aspect of substrate-level phosphorylation in glyeoly
view available hint(s)
an enzyme is required in order for
the reaction to occur
a bond must be broken between an
organic molecule and phosphate
before atp can form
one of the substrates is a molecule
derived from the breakdown of
glucose.
the phosphate group added to adp
to make atp comes from free
inorganic phosphate ions.
the enzymes involved in atp
synthesis must be attached to a
membrane to produce atp
correct statements
incorrect statements

Explanation:

• Correct statements:
• In substrate - level phosphorylation, an enzyme is required. Enzymes like phosphoglycerate kinase and pyruvate kinase are involved in glycolysis for this process.
• A bond between an organic molecule and phosphate must break. For example, in the conversion of 1,3 - bisphosphoglycerate to 3 - phosphoglycerate, the bond between the phosphate and the organic molecule (1,3 - bisphosphoglycerate) breaks, and the phosphate is transferred to ADP to form ATP.
• One of the substrates is a glucose - breakdown product. Glycolysis breaks down glucose, and intermediates like 1,3 - bisphosphoglycerate (derived from glucose breakdown) are substrates in substrate - level phosphorylation.
• Incorrect statements:
• The phosphate for ATP synthesis in substrate - level phosphorylation does not come from free inorganic phosphate ions. It comes from a phosphorylated organic intermediate.
• The enzymes involved in substrate - level phosphorylation (e.g., in glycolysis) are in the cytoplasm (not attached to a membrane). Membrane - attached enzymes are more relevant to oxidative phosphorylation (e.g., in the electron transport chain in mitochondria).

Answer:

• Correct statements:
• An enzyme is required in order for the reaction to occur.
• A bond must be broken between an organic molecule and phosphate before ATP can form.
• One of the substrates is a molecule derived from the breakdown of glucose.
• Incorrect statements:
• The phosphate group added to ADP to make ATP comes from free inorganic phosphate ions.
• The enzymes involved in ATP synthesis must be attached to a membrane to produce ATP.