link: bit.ly/dnareplicationbio
name: harley barnard
*click on \interact and learn!\*
1. dna replication is the process of making _________ copies of dna!
2. _________ are necessary to perform the steps of dna _________.
*select the helicase enzyme*
3. watch the animation, then click continue!
a. what did helicase do to the dna double helix? (you can also write your answer in the observation box)
*select the primase enzyme*
4. watch the animation, then click continue!
a. notice that ________ added rna ________. ________ are needed because dna ___________ iii can only add new ___________ to an existing strand.
*select dna polymerase iii*
5. watch the animation
a. notice that ___ _________ iii added new ___________ to build new dna strands
b. also, notice that dna polymerase can only add to an ________ strand. this is why rna ________ were needed before dna ___________ could start working
c. dna polymerase builds new strands by adding ________ following base pairing rules. ___ pairs with ___ and ___ pairs with ____
d. drag and drop nucleotides to build your new strands!
i. notice that you used ___ ___________ iii to create ___________ molecules of dna by following base - pairing rules
*select dna polymerase i*
6. dna polymerase i removes ___ ___________ and replaces them with ___ nucleotides
*select ligase*
7. after dna polymerase i replaces ___ primers with ___ nucleotides, this new ___________ of dna needs to be attached to the rest of the strand
a. also, for several reasons, new dna strands are sometimes built in fragments, called ___________ fragments.
b. it is necessary to attach the fragments together. this is the job of ________
c. drag the ligase to fix the breaks in the _______ ___________ backbone.

Question

link: bit.ly/dnareplicationbio
name: harley barnard
*click on \interact and learn!\*
1. dna replication is the process of making _________ copies of dna!
2. _________ are necessary to perform the steps of dna _________.
*select the helicase enzyme*
3. watch the animation, then click continue!
 a. what did helicase do to the dna double helix? (you can also write your answer in the observation box)
*select the primase enzyme*
4. watch the animation, then click continue!
 a. notice that ________ added rna ________. ________ are needed because dna ___________ iii can only add new ___________ to an existing strand.
*select dna polymerase iii*
5. watch the animation
 a. notice that ___ _________ iii added new ___________ to build new dna strands
 b. also, notice that dna polymerase can only add to an ________ strand. this is why rna ________ were needed before dna ___________ could start working
 c. dna polymerase builds new strands by adding ________ following base pairing rules. ___ pairs with ___ and ___ pairs with ____
 d. drag and drop nucleotides to build your new strands!
 i. notice that you used ___ ___________ iii to create ___________ molecules of dna by following base - pairing rules
*select dna polymerase i*
6. dna polymerase i removes ___ ___________ and replaces them with ___ nucleotides
*select ligase*
7. after dna polymerase i replaces ___ primers with ___ nucleotides, this new ___________ of dna needs to be attached to the rest of the strand
 a. also, for several reasons, new dna strands are sometimes built in fragments, called ___________ fragments.
 b. it is necessary to attach the fragments together. this is the job of ________
 c. drag the ligase to fix the breaks in the _______ ___________ backbone.

Accepted Answer

# Brief Explanations:
1. DNA replication is the process of making identical copies of DNA.
2. Enzymes are necessary to perform the steps of DNA replication.
3a. Helicase unwinds the DNA double helix.
4a. Primase added RNA primers. Primers are needed because DNA polymerase III can only add new nucleotides to an existing strand.
5a. DNA polymerase III added new nucleotides to build new DNA strands.
5b. DNA polymerase can only add to an existing strand. This is why RNA primers were needed before DNA polymerase could start working.
5c. DNA polymerase builds new strands by adding nucleotides following base - pairing rules. Adenine pairs with Thymine and Cytosine pairs with Guanine.
5di. Notice that you used DNA polymerase III to create new molecules of DNA by following base - pairing rules.
6. DNA polymerase I removes RNA primers and replaces them with DNA nucleotides.
7. After DNA polymerase I replaces RNA primers with DNA nucleotides, this new segment of DNA needs to be attached to the rest of the strand.
7a. Also, for several reasons, new DNA strands are sometimes built in fragments, called Okazaki fragments.
7b. It is necessary to attach the fragments together. This is the job of Ligase.
7c. Drag the ligase to fix the breaks in the sugar - phosphate backbone.

# Answer:
1. identical
2. Enzymes; replication
3a. Unwinds it
4a. Primase; primers; Primers; polymerase; nucleotides
5a. DNA polymerase; nucleotides
5b. existing; primers; polymerase
5c. nucleotides; Adenine; Thymine; Cytosine; Guanine
5di. DNA polymerase; new
6. RNA primers; DNA
7. RNA; DNA; segment
7a. Okazaki
7b. Ligase
7c. sugar - phosphate

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