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lets take a more zoomed - out view of the geography of transcription. dna is double - stranded, so either strand can hold the actual sequence of genetic information required to make a protein or a functioning rna. in any gene, the strand that isread by rna polymerase to make rna - the strand that is transcribed - is called the template strand. the complementary strand, which is not transcribed, is called the non - template (coding) strand. some life scientists refer to the non - template strand in a gene as the coding strand. why is this alternative name logical? the non - template strand has the same nucleotide sequence, or code, as the rna product, except thymine (t) is replaced by uracil (u). the non - template strand has the same nucleotide sequence, or code, as the template strand, except thymine (t) is replaced by uracil (u). rna polymerase reads the non - template strand to determine the nucleotide sequence, or code, in the rna.
The non - template strand is complementary to the template strand. RNA polymerase synthesizes RNA using the template strand. The RNA sequence is complementary to the template strand. Since the non - template strand is complementary to the template strand, it has the same sequence as the RNA (with T in DNA replaced by U in RNA).
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The non - template strand has the same nucleotide sequence, or code, as the RNA product, except thymine (T) is replaced by uracil (U).