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Question
an f factor has a mutation that affects the ability of relaxase to join single - stranded dna. it does not affect the other functions of relaxase. at what stage of conjugation would a cell containing only f factors with this mutation be stuck? multiple choice the f factor would not be cut at the origin of transfer the f factor would not be able to be transferred to the exporter the f factor will be stuck in the conjugation bridge the f factor will move to the recipient cell but will not be circularized
Relaxase is involved in nicking the F - factor at the origin of transfer and also in circularizing the single - stranded DNA in the recipient cell. Since the mutation affects the ability of relaxase to join single - stranded DNA but not other functions, the F - factor will move to the recipient cell but won't be circularized as circularization requires the activity of relaxase to join the ends of the single - stranded DNA.
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The F factor will move to the recipient cell but will not be circularized