Question

exercise 1 - questions
question 1
what are the functions of the lysis solution, salt, and alcohol in the extraction of dna performed in this exercise?

Explanation:

• Lysis solution: Breaks open cells (cell lysis) to release DNA. It may contain detergents (e.g., SDS) that disrupt cell membranes and nuclear membranes.
• Salt: Helps in DNA precipitation. Monovalent cations (e.g., from \(NaCl\)) neutralize the negative charge of the phosphate backbone of DNA. This reduces the repulsion between DNA strands, making them come together.
• Alcohol: Precipitates DNA. DNA is less soluble in alcohol (usually cold ethanol or isopropanol) than in the aqueous - salt - DNA solution. The alcohol dehydrates the DNA molecule, allowing it to aggregate and precipitate out of the solution.

Answer:

• Lysis solution: Breaks open cells to release DNA.
• Salt: Neutralizes DNA's charge for precipitation.
• Alcohol: Precipitates DNA by reducing its solubility.