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Question
different photosynthetic pigments from a plant pigment extract can be separated by
multiple choice
a respirometer.
a capillary tube.
spectrophotometry.
paper chromatography.
Paper chromatography is a technique used to separate mixtures based on the different solubilities and affinities of the components for the mobile and stationary phases. Photosynthetic pigments in a plant extract have different solubilities in the solvent (mobile phase) and different attractions to the paper (stationary phase). As the solvent moves up the paper, the pigments separate. A respirometer measures gas exchange (like oxygen consumption or carbon dioxide production) related to respiration. A capillary tube is a thin tube and not a separation technique. Spectrophotometry measures the amount of light absorbed or transmitted by a substance, used for quantification of substances based on their light - absorbing properties, not for separation.
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paper chromatography.