Question

analyze the information provided and the diagram of transcription in action, then determine which statements are accurate. select all that apply. the transcription machinery has two jobs. the first is to orient an incoming ntp in a way that creates hydrogen bonds with the complementary base in dna. the second is to catalyze the formation of a phosphodiester linkage that adds the ntp to the growing rna strand. transcription is an example of energetic coupling. the polymerization reaction is spontaneous because other reactions add phosphate groups to ribonucleotides, creating ntps with high potential energy. rna is synthesized in the 3’ to 5’ direction. the dna template is
ead\ in the 5’ to 3’ direction, meaning that this is the direction the transcription machinery moves along the dna template.

Explanation:

• First statement: Transcription machinery does orient NTPs for hydrogen - bond formation with DNA bases and catalyzes phosphodiester bond formation for RNA strand elongation. This is a fundamental aspect of transcription.
• Second statement: Transcription involves energetic coupling. The hydrolysis of the high - energy phosphate bonds in NTPs (which are formed by adding phosphate groups to ribonucleotides) drives the non - spontaneous polymerization reaction of RNA synthesis.
• Third statement: RNA is synthesized in the \(5'\) to \(3'\) direction (not \(3'\) to \(5'\)). The new nucleotides are added to the \(3'\) - OH group of the growing RNA strand.
• Fourth statement: The DNA template is “read” in the \(3'\) to \(5'\) direction. RNA polymerase moves along the DNA template in the \(3'\) to \(5'\) direction while synthesizing RNA in the \(5'\) to \(3'\) direction.

Answer:

First statement, Second statement.