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20 multiple choice 1 point in plasmid miniprep, naoh/sds functions to pellet the cells lyse the cells neutralize the cell suspension precipitate plasmid dna
In plasmid miniprep, NaOH (sodium hydroxide) is a strong base and SDS (sodium dodecyl sulfate) is a detergent. Together, they are used to lyse (break open) bacterial cells. Pelleting cells is done by centrifugation, neutralization is done by a subsequent reagent (like potassium acetate), and precipitating plasmid DNA is done by adding alcohol (like isopropanol or ethanol) after neutralization. So the function of NaOH/SDS is to lyse the cells.
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B. lyse the cells (assuming the options are labeled as A. pellet the cells, B. lyse the cells, C. neutralize the cell suspension, D. precipitate plasmid DNA)