QUESTION IMAGE
Question
10 analysis of dna fragments in gel electrophoresis involves
2
points
multiple choice
larger fragments that move slowly and remain closer to the wells
an electric current through the gel that causes dna fragments to migrate
all of the choices are correct
dna fragments that are stained to see them
dna that has an overall negative charge and moves to the positive pole
Gel electrophoresis is a technique used in molecular biology. DNA has a negative charge due to its phosphate backbone. When an electric current is applied through the gel (usually agarose), DNA fragments migrate. Larger fragments move more slowly and stay closer to the wells (where the DNA is initially loaded). DNA fragments are stained (e.g., with ethidium bromide) to visualize them under UV light. Since DNA is negatively charged, it moves towards the positive pole. All the described phenomena (movement due to charge, size - related migration, staining) are part of gel electrophoresis analysis.
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All of the choices are correct